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and HRP enzyme conjugate are sequentially added to microwells pre-coated with a transcription factor E2F1 (E2F1) capture antibody
③ Antibody detection: Centrifuge the dry powder to the bottom of the tube and use 110uL Buffer for dilution ( 1× ) Dissolve and let stand at room temperature 5 Get after minutes 100× Mother liquor
NFHEV Immunogen Recombinant Protein Location Cytoplasm
Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing
Human SALL1 ELISA Kit Size:96T and HRP enzyme conjugate areProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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